SIES Institute of Chromatography and Spectroscopy

Gas Chromatography & Headspace Techniques

Course No - ICS-001
Duration - 1 day

Programme details

The course will cover the basic theory and principles of Gas Chromatography (GC) and its applications. GC Terminology, GC hardware, sample injection techniques, Practical Session (Group assignment for sample analysis) instruments setup, quantitative techniques. Operating software, column efficiency, resolution and troubleshooting. Headspace Gas Chromatography techniques includes hardware, instrument setup, optimization, modes of operation, qualitative and quantitative calculations.

Course Outline

  • Basic theory of Gas Chromatography and HSGC
  • Software and key Applications
  • Essential oil, Pharmaceutical and Petrochemical applications.
  • Practical Demo with hands training

Gas Chromatography & Mass spectrometry Technologies

Course No - ICS-002
Duration - 1 day

Programme details

The course will cover basic theory and principles of Gas Chromatography / Mass Spectrometer (GC/MS) and its applications. Topics covered includes GC/MS Technology, GC/MS hardware, instrument startup and setup, MS tuning, Operating software, developing GC control method and MS control method, spectral data processing and spectral library searching

Course Outline

  • Basic theory of Gas Chromatography and Mass Spectrometers EI and CI modes
  • Full scan, SIM (Selected ion monitoring)
  • Routine Data Acquisition, Spectral Isolation & Library Searching
  • Essential oil, Pharmaceutical and petrochemical applications.
  • Practical Demo with hands training

High Performance Liquid Chromatography

Course No - ICS-003
Duration - 1 day

Programme details

The course will cover the basic theory and principles of liquid chromatography (LC). Topics covered includes different modes of LC such as normal and reversed phase chromatography, isocratic and gradient modes. Hardware components injectors, detectors and pumps. Basic maintenance, quantitative technique and data handling.

Course Outline

  • Theory of Liquid chromatography
  • UV-Vis and DAD detector operations
  • Software operation
  • Analysis of API and formulations
  • Impurity profiling
  • Practical Demo with hands training

Ion Chromatography (IC)

Course No - ICS-005
Duration - 1 day

Programme details

The course will cover the basic theory and principles of Ion Chromatography and its applications. Anion and cation analysis. IC applications in Environmental, Pharmaceutical samples .

Course Outline

  • Chromatography and its terminologies
  • Ion Chromatography theory
  • Detection methods in Ion Chromatography
  • Sample Preparation Techniques in Ion Chromatography
  • Hands- on training on Metrohm Ion Chromatography software MagIC net™
  • Method development in Ion Chromatography
  • Practical Demo with hands training

Fourier Transform Infrared (FTIR) Spectroscopy & UV-Vis spectroscopy

Course No - ICS-004
Duration - 1 day

Programme details

The course will cover the basic theory and principles of UV-Vis and FTIR instrument and their applications. Sample preparations of solids and liquids by both the transmission and reflection techniques.

Course Outline

  • Theory of spectroscopy
  • UV-Vis and IR spectrometer principle of operation
  • Software operations
  • IR sample handling by transmission techniques – KBr disk, liquid cells etc.
  • IR sample handling by reflection techniques – diffuse reflectance and ATR accessories
  • Practical Demo with hands training